中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (50): 9316-9320.doi: 10.3969/j.issn.2095-4344.2012.50.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

芒果苷对大鼠成骨细胞增殖作用的影响

潘生才1，李晓峰2，唐毓金1，谢克恭1，蓝常贡1，卢贤哲1   

  1. 1右江民族医学院附属医院脊柱骨病外科，广西壮族自治区百色市 533000；2 广西医科大学第一附属医院创伤骨科手外科，广西壮族自治区南宁市 
    530021
  • 出版日期:2012-12-09 发布日期:2012-12-09
  • 通讯作者: 唐毓金，硕士，主任医师，右江民族医学院附属医院脊柱骨病外科，广西壮族自治区百色市 533000 tangyujin196709@163.com

Effect of mangiferin on the proliferation of rat osteoblasts

Pan Sheng-cai1, Li Xiao-feng2, Tang Yu-jin1, Xie Ke-gong1, Lan Chang-gong1, Lu Xian-zhe1   

  1. 1Department of Spinal and Bone Disease, the Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China; 2Department of Traumatic Orthopedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Online:2012-12-09 Published:2012-12-09
  • Contact: Tang Yu-jin, Master, Chief physician, Department of Spinal and Bone Disease, the Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China tangyujin196709@163.com

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401

被引次数

2

摘要：

背景：芒果苷以及含芒果苷植物提取物具有多方面生理和药理作用，但其是否促进成骨细胞增殖尚未见报道。

目的：验证芒果苷对体外培养SD大鼠成骨细胞增殖的影响。

方法：取新生的SD大鼠的颅骨，二次酶消化法培养成骨细胞。应用MTT法及细胞周期的流式细胞仪检测，观察不同浓度的芒果苷对大鼠成骨细胞毒性，不同浓度的芒果苷对体外培养成骨细胞增殖的影响。

结果与结论：在一定浓度范围内，不同浓度的芒果苷均可促进成骨细胞增殖、分化，并呈现出一定的量效、时效关系。芒果苷最佳促进增殖浓度为40 μmol/L。药物作用时间48，72 h，芒果苷20，40，80 μmol/L组均有显著促进成骨细胞增殖的作用(P < 0.01或P < 0.05)。芒果苷浓度20，40，80 μmol/L等各组细胞S期比值均较正常细胞显著增高，以芒果苷40 μmol/L浓度组增高最显著(P < 0.05)。表明在一定浓度范围内，芒果苷具有促进体外成骨细胞增殖的作用。

关键词: 芒果苷, 成骨细胞, 细胞增殖, 组织工程, 细胞周期

Abstract:

BACKGROUND: Mangiferin and its extracts have many physiological and pharmacological effects, but whether they can promote the proliferation of osteoblasts has not been reported.
OBJECTIVE: To observe the effects of mangiferin on the proliferation of osteoblasts cultured in vitro from SD rats.
METHODS: The osteoblasts from the skull of neonatal SD rats were cultured by the second enzyme digestion. The MTT method and flow cytometry were used to observe the cytotoxicity proliferation of osteoblasts cultured in vitro by different concentrations of mangiferin at different time points.
RESULTS AND CONCLUSION: In a certain range of concentrations, different concentrations of mangiferin could promote the proliferation and differentiation of osteoblasts in a certain dose and time dependent manner. The optimal concentration of mangiferin was 40 μmol/L to promote the proliferation of osteoblasts. When the drug action time was 48 and 72 hours, 20, 40 and 80 μmol/L mangiferin could significantly promote osteoblast proliferation (P <0.01 or P < 0.05). The S-phase fraction of osteoblasts treated with 20, 40, and 80 μmol/L mangiferin was significantly increased than that of normal cells, especially those treated with 40 μmol/L mangiferin (P < 0.05). These findings indicate that in a certain range of concentrations, mangiferin can promote the proliferation of osteoblasts in vitro.